Device

Part:BBa_K2018030

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-06-16)


No part name specified with partinfo tag. is a PHA secretion system based on the type I secretion system for hemolysin.

It encodes the inner membrane transport complex proteins HlyB and HlyD. These transport proteins, derived from Escherichia coli strain SEC470. The BioBrick also encodes part of the hemolysin toxin, HlyA, fused with phasin, a protein that binds to PHB granules. When this BioBrick is transformed into PHB producing cells, PHB will be secreted. The promoter is a LacL promoter so the expression of the system can be regulated.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4196


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